Opening the doors to Europe’s first specific pathogen free sheep flock at the AO Research Institute Davos

Last Friday, August 27, the preclinical research facility of the AO Research Institute Davos (ARI) proudly opened the doors to its highly anticipated specific pathogen free (SPF) sheep facility which stands directly adjacent to the AO center in Davos.
The pre-inauguration gave invitees a one-time chance to tour the facility, before all areas get disinfected, and it then officially closes to all but the sheep and their caregivers.

Christoph Lindenmeyer, CEO, Geoff Richards, Director of ARI and Executive Director AO Foundation R&D, Daniel Arens, Senior Project Leader, as well as Urban Lanker, Manager Preclinical Facility at ARI were happy to welcome many representatives of the cantonal and local community. These included Jon Domenic Parolini, Regierungsrat at Kanton Graubünden and the Mayor of Davos, Philipp Wilhelm (Landammann). Unfortunately, Stephan Zeiter, Program Manager Preclinical Services and Tim Buchholz, Project Leader, were not able to attend, after having dedicated much time to this project.

All were impressed with the state-of-the-art, 6,575-square-meter zone expansion that is due to house the world’s first SPF flock of sheep outside of North America this September. Beyond the impressive facilities, this expansion fulfils the community’s clear commitment to be global research leaders and continues to put Davos on the map within Science City Davos. This facility and indeed the flock themselves will fulfill an unmet need in Europe and beyond while maintaining the highest principles of animal welfare. It will achieve this, according to Richards, through the use of SPF sheep, and careful breeding, that in turn will reduce and refine animal experiments. "The AO, whenever possible, uses other means to develop new treatments that do not involve animals (in silico, in vitro and ex vivo) and strongly follows 3R principles to reduce, refine and replace animal experiments where possible. Despite this, there is a need to use animals in preclinical research before treatments can be applied to help both human and animal patients. We do this with care, caution, and respect, minimizing the burden on the animals,” Richards said.

Why sheep and what are SPF sheep?
When preclinical research into bone fractures and therapies involving animals is needed to ensure that new treatments are effective and safe, sheep are often selected because the healing of a fracture is very similar in sheep and humans.
SPF animals are better than non-SPF animals for such research, as they are free of certain pathogens that the animal could carry without showing clinical symptoms. Once sheep are part of a study, a disease could break out and either, in the worst case, result in losing the animals, or affecting the outcome of the study, as the sheep would have to fight the disease. Further, certain pathogens can also infect humans. Hence the desire to use SPF sheep in studies.

Animal welfare has always been, and remains, central to the AO and ARI, therefore SPF animals used in research also follows this, as Zeiter explains “Sheep without underlying diseases not only increase the reproducibility and safety for the sheep and humans, but we will use fewer sheep in research because there is less variability involved”.

 

Overcoming the challenge of breeding SPF sheep
Breeding SPF sheep comes with many challenges. Lambs must initially be delivered by caesarean section and thoroughly examined to ensure that they do not have diseases transmitted in the womb or acquired at birth through the birth canal. They then need to be carefully separated from other animals that could carry the pathogens. A biosecurity barrier, quarantine zone, easy-to-clean walls and installations will help keep the necessary hygiene.

During the tour, Lanker, Manager Preclinical Facility at ARI, explained how important both the breeding process as well as designing the unique facilities are, and how they broke new ground in ensuring the highest quality and standards to ensure lambs delivered by C-section are healthy and beyond as they grow.

Breaking ground with the new facility
The facility has ventilation systems that adapt to the temperature conditions outside, while the lighting is also programmed to give natural light cycles, rendering the whole facility as open air and natural as possible including a spacious open-air plan.

During the tour, Lanker demonstrated how the facility is set up to ensure the highest levels of hygiene. Instruments and machines are disinfected beforehand and only used in the hygiene zone, caregivers must also take special precautions for example changing clothes and taking either traditional water or an air shower.